Brains were removed from two male and two female pups on PN0 within 6 h of birth and placed in a Zivic Miller brain mold.
A 3-mm section was cut that contained both the POA and medial basal hypothalamus (MBH) (13).
Once it reaches the brain, testosterone is converted to estradiol by the aromatase enzyme.
Focal adhesion kinase (FAK) and paxillin, both members of the focal adhesion complex family of proteins, were significantly elevated in the newborn female compared with the male hypothalamus.
Sex differences in these proteins were not detected in brain regions that are not subject to substantial organizational effects of steroids.
The anterior commissure was used to guide both the dorsal and lateral borders of the dissection (14).
The two MBH samples were pooled for each sex and homogenized with a polytron in boiling lysate buffer [10 m sodium orthovanadate, 1% sodium dodecyl sulfate] supplemented with 00 phosphatase inhibitor (cocktail I; Sigma-Aldrich, St. All additional Power Blot analysis was performed by BD Biosciences Phar Mingen.
There are sex differences in signal transduction in the neonatal rat brain.